applications, for example, in estimating the volume of blood.

Single dilution method

Consider a natural analyte rich in isotope ⁱA (denoted as A), and the same analyte, enriched in isotope ^jA (denoted as B). Then, the obtained mixture is analyzed for the isotopic composition of the analyte, R_AB = n(ⁱA)_AB/n(^jA)_AB. If the amount of the isotopically enriched substance (n_B) is known, the amount of substance

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