pectinase might typically be activated at 45 to 55 °C and work well at a pH of 3.0 to 6.5.

Reaction pathway

Pectinases depolymerise pectin through hydrolysis, trans-elimination and deesterification reaction processes, breaking down the ester bond that holds together the carboxyl and methyl groups in pectin.

Endo-polygalacturonase progresses through a reaction along the following pathway:

(1,4-alpha-D-galacturonosyl)_n+m + H₂O

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