James VI of Scotland gave the town the status of a Royal Burgh and trading rights, recognizing the importance of the port, called the draucht of Anstruther. The bounds of the new Burgh were the "Silver Dyke" on the east, the low water line on the south, the Anstruther burn to the west, and the Kylrynnie march road.

James Melville's diary provides a graphic account of the arrival of a ship from the Spanish Armada