the cytoplasm that lead to different protein accumulation in the posterior vs. the anterior. Mex-5 accumulates in the anterior while PIE-1 and P granules (see below) accumulate in the posterior.

*Specification of germ line
:P granules were identified as the cytoplasmic determinants. While uniformly present at fertilization, these granules become localized in the posterior P1 cell prior to the first division. These granules are further localized between each division into P cells (ex. P2, P3) until after the fourth division when