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<p>sphaeroides, Rhodopseudomonas palustris, Rhodobacter capsulatus.</p>

<p><big>Cultivation</big></p>
<p>Under the laboratory conditions, extra nitrogen sources are not needed to grow free-living diazotrophs. Carbon sources (such as sucrose or glucose) and a small amount of inorganic salt are added to the medium. Free-living diazotrophs can directly use atmospheric nitrogen (N<sub>2</sub>). However, while cultivating several symbiotic diazotrophs, such as rhizobia, it is necessary to add nitrogen because rhizobia and other symbiotic nitrogen-fixing bacteria</p><p>
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