be clamped successively at different membrane potentials in a single patch. This results in channel activation as a function of voltage, and a complete I-V (current-voltage) curve can be established in only one patch. Another potential drawback of this technique is that, just as the intracellular pathways of the cell are not disturbed, they cannot be directly modified either.

Inside-out patch

In the inside-out method, a patch of the membrane is attached to the patch pipette, detached from

1.Previous
3.Next