recognition sites to serve as sites where foreign DNA may be introduced
* a selectable genetic marker gene that can be used to enable the survival of cells that have taken up vector sequences
* a tag gene that can be used to screen for cells containing the foreign DNA

Preparation of vector DNA

The cloning vector is treated with a restriction endonuclease to cleave the DNA at the site where foreign DNA will be inserted. The restriction enzyme is chosen to generate a configuration at the cleavage site that is compatible

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