Later, on 18 March 1525, Emperor Charles V erected the Province or Governorship of Margarita, granting it as property for two lifetimes to Marcelo Villalobos. When he died on 24 June 1526, he was succeeded by his daughter Aldonza Villalobos Manrique according to the royal capitulation of 13 June 1527, but since she was a minor, her mother Isabel Manrique assumed the government of the island.

Isabel Manrique took care of the settlement task by bringing groups of colonists to settle