Her aunt, Sibill Hampden, married Thomas Hawtrey, whose son William Hawtrey (died 1597), was custodian of Lady Mary Grey at Chequers in 1565.

The genealogical detail of her family is variously reported. Her brothers were Richard Hampden of Dunton and Missenden Abbey, Clerk of the Kitchen to Elizabeth I, Griffith Hampden (MP), who entertained Queen Elizabeth at Hampden House,