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thrombin (factor IIa), an active serine protease. The activation of thrombin is a critical reaction in the coagulation cascade, which functions to regulate hemostasis in the body. To produce thrombin, the prothrombinase complex cleaves two peptide bonds in prothrombin, one after Arg²⁷¹ and the other after Arg³²⁰. Although it has been shown that factor Xa can activate prothrombin when unassociated with the

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