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in detecting and sequencing 5mC in single cells. Treatment of DNA with bisulfite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Therefore, DNA that has been treated with bisulfite retains only methylated cytosines. To obtain the methylome readout, the bisulfite-treated sequence is aligned to an unmodified genome. Whole genome bisulfite sequencing was achieved in single cells in 2014. The method overcomes the loss of DNA associated with the typical procedure, where sequencing adapters are added prior to

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