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Organisms that ferment lactose appear dark/black or green often with "nucleated colonies"--colonies with dark centers. Organisms that do not ferment lactose will appear pink and often mucoid.

This culture media is important in medical laboratories by allowing the identification of enteric bacteria microbes in a short period of time.

*Rapid lactose fermentation produces acids, which lower the pH. This encourages dye absorption by the colonies, which are now colored purple-black.
*Lactose

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