located neural tube
* The externally located epidermis
* The neural crest cells, which develop in the region between the neural tube and epidermis but then migrate to new locations

# Primary neurulation begins after the neural plate forms. The edges of the neural plate start to thicken and lift upward, forming the neural folds. The center of the neural plate remains grounded, allowing a U-shaped neural groove to form. This neural groove sets the boundary between the right and left sides