Flagellate cells however have been secondarily lost in some opisthokont groups, including most of the fungi.

Opisthokont characteristics include synthesis of extracellular chitin in exoskeleton, cyst/spore wall, or cell wall of filamentous growth and hyphae; the extracellular digestion of substrates with osmotrophic absorption of nutrients; and other cell biosynthetic and metabolic pathways. Early opisthokonts were likely amoeboid and phagotrophic.

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