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For mutation screening, SSCP assays are usually designed around short PCR fragments because detection sensitivity falls as amplicon length increases; classic evaluations found an optimum around 150 bp, and review literature commonly describes fragments under 300 bp, preferably under 200 bp, as the practical range. It can be used in genotyping to detect homozygous individuals of different allelic states, as well as heterozygous individuals that should each demonstrate distinct patterns in an electrophoresis experiment. SSCP is also widely used

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