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<p>the vacuole membrane as tonoplast.Christian de Duve discovered mammalian lysosomes using biochemical methods in the mid-1970s. De Duve named lysosomes based on their biochemical properties (from the Greek lysis - digestive and soma - body). Their physical form was confirmed shortly afterward by electron microscopy. Because the lysosome shares many properties with vacuoles across taxonomical kingdoms, the notion that vacuoles and lysosomes are distinctly different organelles is more historical than functional.</p>

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