techniques, in which multiple rounds of photobleaching is done so that the spread of fluorescence loss can be observed in cell.

Loss of activity caused by photobleaching can be controlled by reducing the intensity or time-span of light exposure, by increasing the concentration of fluorophores, by reducing the frequency and thus the photon energy of the input light, or by employing more robust fluorophores that are less prone to bleaching (e.g. Cyanine Dyes, Alexa Fluors