who also married Estienne's mother, the widow Estienne. As Estienne was not yet of age at the time of his father's death, Colines and Gilles Nepveu (the husband or fiancé of his sister Nicole) became his legal guardians. Estienne and Colines likely collaborated in Estienne print shop for a time. Colines was known for his exquisite type cutting, whereas Estienne was known for his accuracy.

In 1526, Robert Estienne assumed control of his father's printing shop while Colines established his own firm nearby. The parties agreed to divide