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<p>for the above is:</p>

<p>RCOO<sup>-</sup> + CoASH + ATP -> RCO-SCoA + AMP + PP<sub>i</sub> <br />This two-step reaction is freely reversible and its <a href="page.php?w=Chemical_equilibrium">equilibrium</a> lies near 1. To drive the reaction forward, the reaction is coupled to a strongly exergonic hydrolysis reaction: the enzyme <a href="page.php?w=pyrophosphatase">inorganic pyrophosphatase</a> cleaves the pyrophosphate liberated from ATP to two phosphate ions, consuming one water molecule in the process. Thus the net reaction becomes:</p>

<p>RCOO<sup>-</sup></p><p>
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