inorganic phosphate. Finally, pyruvate is converted to ethanol and CO₂ in two steps, regenerating oxidized NAD+ needed for glycolysis:
:1. CH₃COCOO^- + H⁺ -> CH₃CHO + CO₂catalyzed by pyruvate decarboxylase
:2. CH₃CHO + NADH + H⁺ -> C₂H₅OH + NAD⁺

This reaction is catalyzed by alcohol dehydrogenase