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is determined by how long the needle remains in the cell. This system typically requires a regulated pressure source, a capillary holder, and either a coarse or a fine micromanipulator. A pulsed flow system, however, allows for greater control and consistency over the amount of sample injected: the most common arrangement for intracytoplasmic sperm injection includes an Eppendorf "Femtojet" injector coupled with an Eppendorf "InjectMan",

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