species O. avosetta is one of a few species known for lining their nest burrows with flower petals. A female might inspect several potential nests before settling in.

Within a few days of mating, the female has selected a nest site and has begun to visit flowers to gather pollen and nectar for her nests; many trips are needed to complete a pollen/nectar provision mass. Once a provision mass is complete, the bee backs into the hole and lays an egg on top of the mass. Then, she creates a partition