meets the sample mixture, and carries it up the plate (elutes the sample). The plate is removed from the container before the solvent reaches the top of the plate; otherwise, the results will be misleading. The solvent front, the highest mark the solvent has travelled along the plate, is marked.

Visualization: The solvent evaporates from the plate. Visualization methods include UV light, staining, and many more.

Separation process and principle

The separation of compounds is due to the differences in their attraction

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