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After ES cells editing, the tetraploid complementation assay can be used to generate genetically modified mouse model (directly produce a viable animal without pronuclear or Blastocyst microinjection.Although the assay was developed and is most widely used in mice, tetraploid complementation approaches have also been applied in other mammals, including rats, where embryonic stem cells have been used to generate viable animals via tetraploid complementation, demonstrating the broader utility

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