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<p> and <a href="page.php?w=herpes_simplex_virus">herpes simplex virus</a>.</p>

<p><big>Physical methods</big></p>
<p>Physical methods are the conceptually simplest, using some physical means to force the transfected material into the target cell's nucleus. The most widely used physical method is <a href="page.php?w=electroporation">electroporation</a>, where short electrical pulses disrupt the cell membrane, allowing the transfected nucleic acids to enter the cell. Other physical methods use different means to poke holes in the cell membrane: <a href="page.php?w=Sonoporation">Sonoporation</a></p><p>
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