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In enzymology, tryptophan 2,3-dioxygenase is a heme enzyme that catalyzes the oxidation of -tryptophan (-Trp) to ''N''-formyl-{{sc, as the first and rate-limiting step of the kynurenine pathway.Tryptophan 2,3-dioxygenase plays a central role in the physiological regulation of tryptophan flux in the human body,

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